NR AVED
AU Mitsuiki,S.; Hui,Z.; Matsumoto,D.; Sakai,M.; Moriyama,Y.; Furukawa,K.; Kanouchi,H.; Oka,T.
TI Degradation of PrPsc by keratinolytic protease from Nocardiopsis sp. TOA-1.
QU Bioscience, Biotechnology, and Biochemistry 2006 May; 70(5): 1246-8
PT journal article
AB A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.
MH Actinomycetales/*enzymology; Animals; Biodegradation; Cricetinae; Hydrogen-Ion Concentration; Male; Peptide Hydrolases/*metabolism; PrPsc Proteins/*metabolism; Temperature
AD Department of Applied Chemistry and Biochemistry, Kyushu Sangyo University, Fukuoka, Japan.
SP englisch
PO Japan