NR AVAQ
AU Everest,S.J.; Thorne,L.T.; Hawthorn,J.A.; Jenkins,R.; Hammersley,C.; Ramsay,A.M.; Hawkins,S.A.C.; Venables,L.; Flynn,L.; Sayers,R.; Kilpatrick,J.; Sach,A.; Hope,J.; Jackman,R.
TI No abnormal prion protein detected in the milk of cattle infected with the bovine spongiform encephalopathy agent
QU Journal of General Virology 2006 Aug; 87(8): 2433-41
PT journal article
AB Milk specimens were collected from lactating cows that had previously been challenged with bovine spongiform encephalopathy (BSE)-infected brain at 4-6 months of age. One group of 10 animals received a single oral dose of 100 g, a second group received 1 g and the third was made up of unexposed controls. The cows were inseminated artificially, and calved at approximately 2 years of age and annually thereafter. Milking was done within the first week following calving and at 10-weekly intervals during the lactation period. Specimens were centrifuged to obtain a fraction enriched for somatic cells and these fractions were analysed for disease-associated, abnormal prion protein (PrP(BSE)) by using a modified commercial BSE ELISA and a different confirmatory assay. No abnormal prion protein has so far been identified in the cell fraction of milk from cattle incubating BSE by using these methods at their limits of detection.
IN Zehn Kuhkälber wurden im Alter von 4-6 Monaten oral mit je 100 g BSE-infektiösem Hirnhomogenat inokuliert, eine weitere Gruppe erhielt Dosen von nur 1 Gramm. Als Färsen wurden diese Tiere genau wie eine nicht inokulierte Gruppe von Negativkontrolltieren künstlich befruchtet. sodass sie im Alter von rund 2 Jahren abkalbten. Man nahm von den Kühen Milchproben, konnte in ihnen aber bis zum Zeitpunkt der Publikation per BSE-ELISA noch kein PrPsc nachweisen.
MH Animals; Blotting, Western; Cattle; Encephalopathy, Bovine Spongiform/*metabolism; Enzyme-Linked Immunosorbent Assay; Female; Milk/*chemistry; Prions/*analysis; Research Support, Non-U.S. Gov't
AD Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK
SP englisch
PO England