NR AUUN
AU Saa,P.; Castilla,J.; Soto,C.
TI Presymptomatic detection of prions in blood
QU Science 2006 Jul 7; 313(5783): 92-4
IA http://www.cwd.cc/Presymptomatic%20Detection%20PRIONS%20IN%20BLOOD%20SOTO%20ET%20AL.pdf
PT evaluation studies; journal article
AB Prions are thought to be the proteinaceous infectious agents responsible for transmissible spongiform encephalopathies (TSEs). PrPsc, the main component of the infectious agent, is also the only validated surrogate marker for the disease, and its sensitive detection is critical for minimizing the spread of the disease. We detected PrPsc biochemically in the blood of hamsters infected with scrapie during most of the presymptomatic phase of the disease. At early stages of the incubation period, PrPsc detected in blood was likely to be from the peripheral replication of prions, whereas at the symptomatic phase, PrPsc in blood was more likely to have leaked from the brain. The ability to detect prions biochemically in the blood of infected but not clinically sick animals offers a great promise for the noninvasive early diagnosis of TSEs.
IN Die Autoren konnten unter Verwendung der von Castilla et al. [AVIO,2006] ausführlich dargestellten PMCA-Technik mit 7 mal 144 Zyklen PrPsc noch nicht nach 14 Tagen und nicht mehr nach 80 Tagen, aber vor allem 20 und 40 Tage, auch noch 60 Tage und ganz schwach noch 70 Tage nach der Inokulation im Blut Scrapie-infizierter Hamster nachweisen. Als die Hamster dann nach rund 110 Tagen erkrankten, war PrPsc in ihrem Blut wieder in relativ großer Menge nachweisbar.
MH Animals; Blood-Brain Barrier; Brain Chemistry; Creutzfeldt-Jakob Syndrome/blood/diagnosis; Cricetinae; Humans; Lymphocytes/chemistry; Lymphoid Tissue/chemistry; Mesocricetus; PrPsc Proteins/*blood/chemistry; Protein Folding; Research Support, N.I.H., Extramural; Scrapie/*blood/*diagnosis; Sensitivity and Specificity; Time Factors
AD George and Cynthia Mitchell Center for Alzheimer's Disease Research, Department of Neurology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0646, USA
SP englisch
PO USA