NR AUUF
AU Lee,D.C.; Sakudo,A.; Kim,C.K.; Nishimura,T.; Saeki,K.; Matsumoto,Y.; Yokoyama,T.; Chen,S.G.; Itohara,S.; Onodera,T.
TI Fusion of Doppel to octapeptide repeat and N-terminal half of hydrophobic region of prion protein confers resistance to serum deprivation
QU Microbiology and Immunology 2006; 50(3): 203-9
PT journal article
AB Our previous studies have shown an essential role played by the octapeptide repeat region (OR) and the N-terminal half of hydrophobic region (HR) in the anti-apoptotic activity of prion protein (PrP). As PrP-like protein Doppel (Dpl), which structurally resembles an N-terminally truncated PrP, did not show any anti-apoptotic activity, we examined apoptosis of HpL3-4 cells expressing Dpl fused to various lengths of the N-terminal region of PrP to investigate whether the PrP/Dpl fusion proteins retain anti-apoptotic function. HpL3-4 cells expressing Dpl fused to PrP(1-124) with the OR and N-terminal half of HR of PrP showed anti-apoptotic function, whereas Dpl fused to PrP(1-95) with OR did not rescue cells from apoptotic cell death induced by serum deprivation. These results indicate that the OR and N-terminal half of HR of PrP retains anti-apoptotic activity similar to full-length PrP.
MH Animals; Apoptosis/*drug effects; Cell Line; Culture Media; Hydrophobicity; Mice; Microsatellite Repeats/*genetics; Neurons/cytology/metabolism/*physiology; PrPc Proteins/chemistry/genetics/*physiology; Prions/*genetics/metabolism/pharmacology; Recombinant Fusion Proteins/chemistry/genetics/metabolism; Research Support, Non-U.S. Gov't; Serum/metabolism; Transfection
AD Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
SP englisch
PO Japan