NR AUTA
AU Schulz,G.; Heit,A.; Hammerschmidt,F.; Gilch,S.; Maas,E.; Wagner,H.; Schätzl,H.M.
TI Vaccination approaches against prion infections
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Therapie THE-06
PT Konferenz-Poster
AB Prion diseases are neurodegenerative infectious disorders for which no prophylactic regimens are known. In order to induce antibodies/auto-antibodies directed against surface-located PrPc, we used a covalently linked dimer of mouse prion protein expressed recombinantly in E. coli. Employing dimeric PrP as an immunogen in combination with adjuvant CpG, we were able to effectively overcome auto-tolerance against murine PrP in PrP wild-type mice without inducing obvious side effects. Treatment of prion-infected mouse cells with polyclonal anti-PrP antibodies generated in rabbit or auto-antibodies produced in mice significantly inhibited the endogenous PrPsc synthesis. In addition, we found immune responses against different epitopes when comparing antibodies induced in rabbits and PrP wild-type mice. Only in the auto-antibody situation in mice an immune reaction against a region of PrP is found that was reported to be involved in the PrPsc conversion process. Our data clearly show that we also induce a Th1-type T-cell response in this auto-immunization situation. Studies on improved antigen application and the efficacy of tolerance breakers like anti-OX40 antibody are ongoing. Our data point to the possibility of developing an active immunoprophylaxis against prion diseases.
AD Gunnar Schulz, Franziska Hammerschmidt (geb. Wopfner), Sabine Gilch, Elke Maas and Hermann M. Schätzl, Institute of Virology, Prion Research Group, Trogerstr. 30, 81675 Munich, Germany; Antje Heit, Hermmann Wagner, Institute of Medical Microbiology and Immunology, Technical University Munich
SP englisch
PO Deutschland
EA Photo des in Greifswald gezeigten Posters, welches nach Auskunft von Prof. Schätzl aktueller als der Tagungsband ist, in dem Frau Hammerschmidt noch unter ihrem Mädchennamen Wopfner aufgeführt ist und die Coautoren Heit, Maas und Wagner noch fehlen, Details
OR Tagungsband