NR AUSU
AU Yan,Z.X.; Stitz,L.; Gaedt,S.; Roth,K.; Mauz,P.S.
TI A new test procedure for evaluation of Prion removal
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Diagnostik DIA-10
PT Konferenz-Poster
AB
We had developed a proof method for contamination and remaining contamination on surgical instruments known as "radionuclide method". After a mock contamination of the instruments with radioactively labelled blood, the instruments are to be viewed on a gamma camera before and after the cleaning. That provides a simple and a non destructive way to localize and measure the remaining contamination both pin-pointedly and quantitatively, which is especially useful for study of reprocessing those instruments with channels and cavities such as endoscopic instruments.
Pathological prion protein, the agent that causes transmissible spongiform encephalopathies (TSE), is found to be able to bind to surgical steel surface and hard to be cleaned away; therefore, which may pose a potential threat of iatrogenic transmission. In searching of new chemical and physical decontamination procedures, question arised has been whether the prion proteins are washed away, denatured or destroyed from surface of an instrument. To answer the question we adapted "radionuclide method" in such a way that purified pathological prion proteins are radioactively labelled and the labelled prion proteins will be used as soil to contaminate test instrument. For the first step the test instrument shall be surgical steel wires. After reprocessing and remaining gamma count measuring, the wires will be used later in a Bioassay to see whether there is any correlation between gamma counts and surviving time of the test animals. If a correlation could be established, then the answer could be found about the nature of a reprocessing procedure against pathological prion proteins on the surface of the test instrument.
AD Klaus Roth, Z.X.Yan, SMP GmbH, Prüfen Validieren Forschen, Service für Medizinprodukte, Paul-Ehrlich-Strasse 40, 72076 Tübingen, Tel. +49 (0)7071/770-4242 ,Fax +49 (0) 7071 770 4244, E-mail: info@smpgmbh.com; L.Stitz, Friedrich Loeffler Institut; Paul-Ehrlich-Strasse 28; 72076 Tuebingen; S.Gaedt, P.S.Mauz, Hals Nasen Ohren Klinik der Eberhard-Karls Universität; Elfriede Aulhorn-Strasse 5; 72076 Tuebingen
SP englisch
PO Deutschland
EA Übersicht und zweite Übersicht von Ingrid
OR Tagungsband