NR AUSM
AU Blasche,T.; Schettler,E.; Balachandran,A.V.; Langenberg,J.A.; Miller,M.W.; Eschenbacher-Kaps,I.; Gerst,K.; Meussdoerffer,F.; Risch,K.; Streich,W.J.; Steinbach,F.; Frölich.K.
TI Surveillance of TSE in cervids from Germany and analysis of rapid tests for detection of cervid PrP
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Diagnostik DIA-02
PT Konferenz-Poster
AB
Transmissible spongiform encephalopathies (TSE) occur in a number of animal species. Chronic wasting disease (CWD) has emerged as an important TSE of captive and free-ranging cervids in North America, naturally affecting several species including mule deer (Odocoileus hemionus), white- tailed deer (Odocoileus virginianus), Rocky Mountain elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi). We performed the recently largest active survey on TSE in German cervids and investigated 4250 roe deer (Capreolus capreolus), 1416 red deer (Cervus elaphus elaphus) and 1390 fallow deer (Dama dama) between 2002 and 2005. All cervids (n=7056) were tested negative for TSE. This survey provides no evidence for the existence of prion diseases in free-living German cervids.
As survey and possible eradication strategies rely on the tests performed, we compared various commercially available rapid TSE tests for their analytical sensitivity against PrP in cervids. Positive control samples (confirmed by PrP immunohistochemistry) originated from CWD cases of North America. Additionally, we tested brain (n=6) and lymph node (n=6) samples from roe deer, red deer and fallow deer within Germany, without applying the Protease K digestion step. Three ELISA-based tests (Bio-Rad TeSeE, TeSeE sheep/goat, Prionics-Check LIA), two Western blot tests (Bio-Rad TeSeE Western Blot and Prionics-Check WESTERN) and one immunochromatographic assay (Prionics-Check PrioSTRIP) were evaluated. To enhance sensitivity, the tests were modified in consultation with manufacturers. Western blot systems were used as reference to compare dilutions of PrP. All six tests were suitable to detect PrP of cervids from all species tested. However, analytical sensitivity demonstrated significant differences between the tests. So we achieved lowest PrPres quantitative sensitivity for the Prionics-Check LIA and figured the BioRad TeSeE Western blot to be the most sensitive test for detection of PrPres in cervids.
AD Tina Blasche, Elvira Schettler, Wolf Jürgen Streich, Falko Steinbach, Kai Frölich, Institute Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany; Aru Balachandran, Canadian Food Inspection Agency, Ottawa, Canada; Julie Langenberg, Wisconsin Department of Natural Resources, Madison, Wisconsin, USA; Michael W. Miller, Colorado Division of Wildlife, Fort Collins, Colorado, USA; Iris Eschenbacher-Kaps, Cenas AG, Kulmbach, Germany; Kirsten Gerst, Kirsten Risch, Landesveterinär- und Lebensmitteluntersuchungsamt MV, Rostock, Germany; Franz Meussdoerffer, University of Bayreuth, Chair of Microbiology, Bayreuth, Germany
SP englisch
PO Deutschland
OR Tagungsband