NR AUSA
AU Bach,C.; Greenwood,A.D.; Horsch,M.; Gilch,S.; Beckers,J.; Erfle,V.; Leib-Mösch,C.; Schätzl,H.M.; Vorberg,I.
TI Differential gene expression in prion infected cell culture versus uninfected cells
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Pathogenese/Infektion PATH-01
PT Konferenz-Poster
AB The underlying pathogenic mechanisms of prion infection are not well characterized. RNA expression profiles for microglia and total brain for Creutzfeldt-Jakob disease and scrapie in mice have been determined. To study the effect of prion infection on gene expression in neuronal cell cultures, a neuroblastoma (N2a) cell clone was infected with either the mouse adapted prion strain 22L or exposed to uninfected brain homogenate as a negative control. Large scale expression analysis was performed using a cDNA microarray chip comprising about 21,000 spotted ESTs. Real-Time PCR was used for confirmation of the microarray data and for semi-quantitative analysis. Over hundred genes were identified that are differentially expressed in 22L-infected cells when compared to uninfected cells. Several of the identified changes in gene expression have also been reported for other neurodegenerative diseases such as Alzheimer's disease.
AD Christian Bach, Sabine Gilch, Hermann Schätzl, Ina Vorberg, Institute of Virology/Prion Research Group, Technical University of Munich, Biedersteinerstrasse 29, 80802 Munich, Germany; Alex Greenwood, Christine Leib-Mösch, GSF-National Research Center for Environment and Health, Institute of Molecular Virology, Neuherberg, Germany; Marion Horsch, Johannes Beckers, GSF-National Research Center for Environment and Health, Institute of Experimental Genetics, Neuherberg, Germany; Volker Erfle, Institute of Virology, Technical University of Munich, Trogerstrasse 4b, Munich, Germany
SP englisch
PO Deutschland
OR Tagungsband