NR AURD
AU Bodemer,W.; Kunze,S.; Lemke,K.; Metze,J.; Bloukas,G.; Kotta,K.; Panagiotidis,C.H.; Sklaviadis,T.K.
TI Atomic Force Microscopy to investigate heavy metal ions as modulator of prion protein binding
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Struktur und molekulare Mechanismen MOL-01
PT Konferenz-Poster
AB The conformational transition of cellular prion protein to an isomer with high beta-sheet content is believed to be associated with transmissible spongiform encephalopathies. However, the function of the prion protein itself in mammalian cells is still under discussion. Due to its copper binding via its octarepeat motif a regulatory function of PrP for heavy metal ions would be plausible. The binding of heavy metal ions to the prion protein was the scientific basis for PrP immobilization on a solid support in order to monitor binding events by atomic force microscopy. Recombinant prion protein was immobilized on mica with addition of copper(II) or nickel(II) ions and without any heavy metal ion as reference. The addition of copper(II) resulted in an enhanced PrP immobilization whereas nickel(II) reduced the surface coverage with PrP. High-resolution data of dried PrP preparations provided for the first time a distribution of geometrical parameters of PrP precipitates, indicating 30 nm3 as the volume of a single PrP molecule. Molecular dynamics simulations were performed to complete the AFM investigation from a theoretical and structural point of view. The calculated volume by molecular modelling software of 33 nm3 is very close the experimentally determined one. Our study belongs to the first ones aiming at an in situ imaging of individual PrP molecules under stringent experimental conditions. AFM proved to be the method of choice as it has already been shown in APP/Aß and alpha-synuclein research.
AD Stefan Kunze, Karen Lemke, Dr.-Ing. Josef Metze (josef.metze@iba-heiligenstadt.de), Institute for Bioprocessing and Analytical Measurement Techniques e.V., Rosenhof, D-37308 Heilbad Heiligenstadt, Germany; G. Bloukas, Konstantina Kotta (ntina_kotta@yahoo.com), Cynthia H. Panagiotidis, Theodoros K. Sklaviadis (sklaviad@auth.gr), Laboratory of Pharmacology, School of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Thessaloniki 541 24, Greece; Prof. Dr. rer. nat. Walter Bodemer (bodemer@dpz.gwdg.de), German Primate Center, Department of Infectious Pathology, Kellnerweg 4, D-37077 Göttingen, Germany
SP englisch
PO Deutschland
EA Photo des in Greifswald erneut gezeigten Posters von der internationalen Tagung in Düsseldorf
OR Tagungsband