NR AUQU
AU Deslys,J.P.
TI Detection and inactivation of BSE and scrapie
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Vortrag V-13
PT Konferenz-Vortrag
AB
The unique resistance of BSE and scrapie agents to usual decontamination techniques and the difficulties of detection poses a very difficult infection control challenge to food industry potentially exposed to the risk, to the pharmaceutical industry and also to health-care facilities.
New diagnostic methods applicable to blood begin to be announced but still need a long process of development and independant evaluation before practical application. For BSE, post-mortem testing at slaughterhouse with rapid tests already constitutes a very efficient method to protect the food chain and consumer.
Concerning decontamination, the drastic procedures recommended (1M sodium hydroxyde or 2% sodium hypochlorite for one hour, 134° autoclaving for 18 min) are not applicable in numerous situations. To implement new techniques, a rapid evolution of the recommendations implies a consensus on the validation methods which will be acceptable by health authorities. Different experimental models have been compared to evaluate the efficiency of classical prion decontamination methods versus new original procedures compatible with fragile devices. Reproducible results have been obtained in a 263K hamster model and a BSE mouse model of intracerebral infection by contaminated wires exposed to different treatments. We observed that limited concentrations of alcali or stabilized bleach, when properly formulated with detergents and used at specific temperatures, could exhibit similar efficiency on prions than the classical NaOH or NaOCl treatments. Short time exposures and compatibility with material render these treatments adapted for practical use. Similarly a dry system based on vaporized hydrogen peroxide under vacuum turned out to be more efficient than autoclaving whilst beeing compatible with electronic devices. These experimental models combined with in vitro protocols based on the detection of PrPres allow to investigate the effectiveness of new versus standard treatments and to explore the mechanisms of action involved.
Our conclusion is that new efficient procedures of prion decontamination can be evaluated in standardized experimental validation systems. As new protocols arise they should be considered for recommentation by official guidelines.
AD Jean-Philippe Deslys (MD, PhD), CEA/DSV/DRM/GIDTIP, 18 route du Panorama, 92265 Fontenay-aux-Roses, France
SP englisch
PO Deutschland
OR Tagungsband