NR AUQD
AU Wenisch,S.; Lücker,E.; Eigenbrodt,E.; Bülte,M.; Leiser,R.
TI Procedures for the unwanted ingredients in meat products with regard to BSE - 4. Histological and immunohistological procedures for the detection of central nervous tissue in meat products
QU Fleischwirtschaft 2000 Jul 12; 80(7): 69-72
PT article
AB Suitable analytical methods are urgently needed with respect to assessment and control of human exposure risk to bovine spongiform encephalopathy. Among these, methods for the detection of specified risk material (SRM), especially of tissues of the central nervous system (CNS), are currently of great interest. In this study we present an immunohistochemical procedure for the detection of central nervous tissue in meat products, Cooked sausages with varying additions of bovine brain and a 'Bregenwurst' containing 15% of porcine brain were produced to serve as reference material. Immunostaining with monoclonal antibodies against neuron specific enolase (gamma gamma-enolase) specifically revealed the central nervous tissue added. Within the positive reference material immunostaining was homogeneously distributed, whereas particles of connective tissue and fragments of striated muscles revealed no signs of immunoreaction. Due to the present results immunohistochemical detection of central nervous tissues by means of the glial fibrillary acidic protein as well as routine food histology must be regarded as inadequate methods. In general, however, routine histological methods might play a central and basic role for the detection of non-neuronal SRM, especially of the lymphoreticular system.
SP deutsch
PO Deutschland