NR AUNQ
AU Halimi,M.; Dayan-Amouyal,Y.; Kariv-Inbal,Z.; Friedman-Levi,Y.; Mayer-Sonnenfeld,T.; Gabizon,R.
TI Prion urine comprises a glycosaminoglycan-light chain IgG complex that can be stained by Congo red
QU Journal of Virological Methods 2006 May; 133(2): 205-10
PT journal article
AB Light chain IgG, a known amyloidotic protein, is present in the urine of prion disease affected individuals in a protease resistant form. In addition, it was shown recently that prion urine samples comprise a significant excess of glycosaminoglycans. Since amyloidotic proteins and glycosaminoglycans are the major components of amyloid aggregates, a Congo red dot blot assay was developed for detection of Creutzfeldt-Jakob disease (CJD) in urine. This assay was also positive for about 10% of patients suffering from diseases such as Alzheimer disease, cerebrovascular attacks and multiple sclerosis, but negative for healthy controls. Both glycosaminoglycans and proteins such as light chain IgG were required for the binding of Congo red to the urine fractions, as shown by the fact that Proteinase K digestion of the samples either after guanidine or after choindrotinase abolished the Congo red signal from the CJD samples.
MH Carrier Proteins/drug effects/*urine; Case-Control Studies; Coloring Agents/*pharmacology; Congo Red/*pharmacology; Creutzfeldt-Jakob Syndrome/*urine; Glycosaminoglycans/*urine; Humans; Prions/chemistry/*urine; Protein Binding/drug effects; Specimen Handling
AD Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem, Israel.
SP englisch
PO Niederlande