NR AUJK
AU Stengel,A.; Bach,C.; Vorberg,I.; Frank,O.; Gilch,S.; Lutzny,G.; Seifarth,W.; Erfle,V.; Maas,E.; Schätzl,H.M.; Leib-Mösch,C.; Greenwood,A.D.
TI Prion infection influences murine endogenous retrovirus expression in neuronal cells
QU Biochemical and Biophysical Research Communications 2006 May 12; 343(3): 825-31
PT journal article
AB Prions as causative agents of transmissible spongiform encephalopathies have been well investigated in experimental and modelling work. However, little is known about the molecular pathogenesis of prion-induced encephalopathies, the role of co-factors, and the interaction of prions with cellular components. We investigated the influence of prion infection on expression of murine endogenous retroviruses (ERVs), which compose approximately 10% of the mouse genome. Hypothalamic neuronal cells (GT1) and neuroblastoma cells (N2a) were examined. Both cell lines can be persistently infected with mouse adapted prion strains, i.e., RML. Using a mammalian retrovirus-specific DNA microarray and quantitative PCR methods, we compared the expression profiles of ERVs in prion-infected, uninfected, and anti-prion compound-treated murine neuronal cell lines, including clonal cell populations. The results suggest that prion infection influences ERV expression in neuronal cell lines, that this influence is cell line-specific, ERV-specific, and responsive to anti-prion compound treatment.
MH Animals; Cell Line; Endogenous Retroviruses/genetics/*metabolism; Mice; Neurons/*virology; Oligonucleotide Array Sequence Analysis; Pentosan Sulfuric Polyester/pharmacology; Prions/*pathogenicity; RNA-Directed DNA Polymerase/analysis; Research Support, Non-U.S. Gov't; Reverse Transcription; Virion/enzymology
AD Institute of Molecular Virology, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D-85764 Neuherberg, Germany.
SP englisch
PO USA