NR AUHS
AU Herrmann,L.M.; Baszler,T.V.; O'Rourke,K.I.; Suarez,C.E.; Bakko,M.; Alverson,J.; Knowles,D.P.
TI Fewer PrPc myeloid-based cells in sheep with the prion-resistant genotype
QU Neuroreport 2006 Feb 6; 17(2): 125-9
PT journal article
AB Numerous sheep with the prion gene (PRNP) that encodes for 171QQ develop scrapie, a neurodegenerative prion disease of sheep. Relatively few cases of scrapie-affected sheep with PRNP genetics that encode for 171RR, however, have been found. Using flow cytometric analysis, statistically fewer PrPc-positive microglia and monocytes were observed from sheep with 171RR PRNP genetics than from sheep with 171QQ PRNP genetics (P<0.05). One possibility for the lack of PrPsc accumulation in brains and lymph nodes of scrapie-exposed sheep with 171RR PRNP genetics is because of fewer PrPc-positive myeloid-derived cells available for conversion of PrPc to PrPsc.
MH Animals; Animals, Newborn; Cells, Cultured; Comparative Study; Female; Flow Cytometry/methods; Genotype; Glial Fibrillary Acidic Protein/metabolism; Immunohistochemistry/methods; Microglia/pathology/ultrastructure; Monocytes/metabolism; Myeloid Cells/*metabolism/ultrastructure; Plant Lectins/metabolism; PrPc Proteins/*metabolism/pathogenicity; Prions/*genetics; RNA, Messenger/biosynthesis; Reverse Transcriptase Polymerase Chain Reaction/methods; Scrapie/genetics/*metabolism/*pathology; Sheep
AD Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, USA. lherrman@vetmed.wsu.edu
SP englisch
PO England