NR AUHI
AU DeJoia,C.; Moreaux,B.; O'Connell,K.; Bessen,R.A.
TI Prion infection of oral and nasal mucosa
QU Journal of Virology 2006 May; 80(9): 4546-56
PT journal article
AB Centrifugal spread of the prion agent to peripheral tissues is postulated to occur by axonal transport along nerve fibers. This study investigated the distribution of the pathological isoform of the protein (PrPsc) in the tongues and nasal cavities of hamsters following intracerebral inoculation of the HY strain of the transmissible mink encephalopathy (TME) agent. We report that PrPsc deposition was found in the lamina propria, taste buds, and stratified squamous epithelium of fungiform papillae in the tongue, as well as in skeletal muscle cells. Using laser scanning confocal microscopy, PrPsc was localized to nerve fibers in each of these structures in the tongue, neuroepithelial taste cells of the taste bud, and, possibly, epithelial cells. This PrPsc distribution was consistent with a spread of HY TME agent along both somatosensory and gustatory cranial nerves to the tongue and suggests subsequent synaptic spread to taste cells and epithelial cells via peripheral synapses. In the nasal cavity, PrPsc accumulation was found in the olfactory and vomeronasal epithelium, where its location was consistent with a distribution in cell bodies and apical dendrites of the sensory neurons. Prion spread to these sites is consistent with transport via the olfactory nerve fibers that descend from the olfactory bulb. Our data suggest that epithelial cells, neuroepithelial taste cells, or olfactory sensory neurons at chemosensory mucosal surfaces, which undergo normal turnover, infected with the prion agent could be shed and play a role in the horizontal transmission of animal prion diseases.
IN Bei mit dem Hamster-adaptierten HY-Erregerstamm der übertragbaren spongiformen Nerzenzephalopathie (TME) intrazerebral inokulierten Hamstern fanden 2005 DeJoia et al., dass PrPsc offenbar durch Nervenbahnen vom Gehirn aus in die Riechschleimhaut und in die Geschmacksknospen der Zunge wandern, von wo aus sie möglicherweise in die Umwelt abgegeben werden und horizontale Übertragungen ermöglichen können.
MH Animals; Biological Markers; Cricetinae; Mesocricetus; Microscopy, Confocal; Mouth Mucosa/*metabolism/*pathology; Nasal Mucosa/*metabolism/*pathology; Prion Diseases/*metabolism/*pathology; Prions/*metabolism/pathogenicity; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
AD Department of Veterinary Molecular Biology, P.O. Box 173610, Montana State University, Bozeman, Montana 59717, USA
SP englisch
PO USA