NR AUFZ
AU Konturek,P.C.; Bazela,K.; Kukharskyy,V.; Bauer,M.; Hahn,E.G.; Schuppan,D.
TI Helicobacter pylori upregulates prion protein expression in gastric mucosa: a possible link to prion disease.
QU World Journal of Gastroenterology 2005 Dec 28; 11(48): 7651-6
IA http://www.wjgnet.com/1007-9327/11/7651.asp
PT journal article
AB AIM: Pathological prion protein (PrPsc) is responsible for the development of transmissible spongiform encephalopathies (TSE). While PrPsc enters the organism via the oral route, less data is available to know about its uptake and the role of gastrointestinal inflammation on the expression of prion precursor PrPc, which is constitutively expressed in the gastric mucosa. METHODS: We studied PrPc expression in the gastric mucosa of 10 Helicobacter pylori-positive patients before and after successful H pylori eradication compared to non-infected controls using RT-PCR and Western blotting. The effect of central mediators of gastric inflammation, i.e., gastrin, prostaglandin E(2) (PGE(2)), tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1ß) on PrPc expression was analyzed in gastric cell lines. RESULTS: PrPc expression was increased in H pylori-infection compared with non-infected controls and decreased to normal after successful eradication. Gastrin, PGE(2), and IL-1ß dose-dependently upregulated PrPc in gastric cells, while TNF-alpha had no effect. CONCLUSION: H pylori infection leads to the upregulation of gastric PrPc expression. This can be linked to H pylori induced hypergastrinemia and increased mucosal PGE(2) and IL-1ß synthesis. H pylori creates a milieu for enhanced propagation of prions in the gastrointestinal tract.
IN Infektionen mit Helicobacter pylori haben bei betroffenen Menschen eine Steigerung der Expression des normalen zellulären Prionproteins PrPc in der Magenschleimhaut zur Folge. Den selben Effekt erzielten die Autoren bei Zelllinien konzentrationsabhängig mit den Entzündungsmediatoren Gastrin, PGE(2) und IL-2ß.
MH Adult; Aged; Cell Line; Gastric Mucosa/*metabolism; Helicobacter Infections/*metabolism; Helicobacter pylori/*pathogenicity; Humans; Interleukin-1/pharmacology; Middle Aged; Prion Diseases/*etiology; Prions/*biosynthesis; RNA, Messenger/analysis; Research Support, Non-U.S. Gov't; Tumor Necrosis Factor-alpha/pharmacology; Up-Regulation
AD Department of Medicine I, University Erlangen-Nuremberg, Germany. peter.konturek@med1.imed.uni-erlangen.de
SP englisch
PO China