NR AUFJ
AU Horlacher,S.; Simon,P.; Bülte,M.
TI Determination of the CNS content and the animal species in retail meat products
OT Bestimmung des ZNS-Gehaltes und der Tierart in Fleischerzeugnissen
QU Fleischwirtschaft 2001 Dez 17; 81(12): 107-8
PT article
AB Tissue of the central nervous system (CNS) are considered as main source of infection with BSE. For the detection of CNS in retail meat products the integrated detection method (INV) based on western blot was used in this study. 18.6 % of 220 field samples indicated central nervous tissue. These industrially samples were manufactured usually using mechanically deboned meat. In none of the them we could detect cattle tissue with the PCR-RFLP. Regarding consumer expectation it should be considered whether the processing of mechanically deboned meat from spinal columns - independent of the animal species - it is forbidden or an appropriate labelling of the products as well as the development of animal species-specific CNS detection methods should be aimed. The detection of CNS proportions exclusively based on NSE led in 37 cases to a false-positive result, i.e. a confirmation with the second, highly specific marker GFAP did not succeed. Among them there were 23 (50 %) poultry meat products as well as 14 (13.5 %) pork products. Future comparative investigations with new CNS marker, in development at the present time, could supply an explanation about the 'real' share in false-positive results.
SP deutsch
PO Deutschland