NR ATVK
AU Lipp,O.
TI [Study on the genetic variability of the porcine prion protein gene]
OT Untersuchung der genetischen Variabilität des porzinen Prionproteingens
QU Inaugural ? Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München von Ortrun Lipp aus Mühlacker, München 13. Februar, 2004
IA http://edoc.ub.uni-muenchen.de/archive/00001946/
PT Dissertation
AB
The aim of the study was to examine a possible variation of the prion protein gene of various pig breeds. Genetic variability of the prion protein gene in sheep corresponds to distinct susceptibilities of the various genotypes.
Limited inoculation experiments in pigs revealed a general susceptibility of pigs after intracerebral but not after oral inoculation.
Sixty six pigs from twelve different breeds were examined. These included the most common breeds in Germany (Deutsche Landrasse, Deutsches Edelschwein, Leicoma, Pietrain, Duroc, Schwäbisch Hällisches Schwein, Bunte Bentheimer), as well as wild boars, two English breeds - Hampshire and Large White -, Belgische Landrasse and Chinese Meishan.
Blood samples were used for analysis, the wild boars were analysed by using liver samples.
DNA was isolated from the blood and liver samples and amplified by PCR (polymerase chain reaction). Primers for PCR were taken from the publication by Martin et al. (1995), and sequencing primers were designed with the help of the program OLIGO(r) version 4.1.
The coding region of the porcine prionprotein gene was amplified, purified and directly sequenced.
The sequences obtained were aligned to the published sequence (Martin et al., 1995), all sequences were found to be identical to the published sequence.
Among the examined pig breeds no genetic variability of the porcine prion protein gene was observed. In contrast to the ovine, murine and human prion protein gene the porcine prion protein gene appears homogeneous.
AD Ortrun Lipp aus dem Zentralinstitut des Tiergesundheitsdienstes Bayern e. V., Poing, der Klinik für Schweine der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München und dem Institut für Tierhygiene und Öffentliches Veterinärwesen der Veterinärmedizinischen Fakultät der Universität Leipzig
SP deutsch
PO Deutschland