NR ATUW
AU Sun,G.; Guo,M.; Shen,A.; Mei,F.; Peng,X.; Gong,R.; Guo,D.; Wu,J.; Tien,P.; Xiao,G.
TI Bovine PrPc directly interacts with alphaB-crystalline
QU FEBS Letters 2005 Oct 10; 579(24): 5419-24
PT journal article
AB We used a bovine brain cDNA library to perform a yeast two-hybrid assay with bovine mature PrPc as bait. The screening result showed that alphaB-crystalline interacted with PrPc. The interaction was further evaluated both in vivo and in vitro with different methods, such as immunofluorescent colocalization, native polyacrylamide-gel electrophoresis, and IAsys biosensor assays. The results suggested that alphaB-crystalline may have the ability to refold denatured prion proteins, and provided first evidence that alphaB-crystalline is directly associated with prion protein.
MH Animals; Cattle; Crystallins/*metabolism; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; PrPc Proteins/*metabolism; Protein Binding; Protein Folding; Research Support, Non-U.S. Gov't; Two-Hybrid System Techniques
AD The Modern Virology Research Centre and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, PR China.
SP englisch
PO Niederlande