NR ATSM
AU Uelhoff,A.
TI Polarisierter Transport des Prion-Proteins
QU Dissertation zum Erwerb des Doktorgrades der Humanbiologie an der Medizinischen Fakultät der Ludwig-Maximilians-Universität zu München, vorgelegt von Armgard Uelhoff aus Recklinghausen, München, 19.07.2005
IA http://edoc.ub.uni-muenchen.de/archive/00004016/
PT Dissertation
AB Several proteins linked to neurodegenerative diseases, such as the -amyloid precursor protein, amyloid -peptide, -secretase, and tau, undergo selective polarized sorting. We investigated polarized sorting of the mammalian prion protein (PrPc) and its homologue doppel (Dpl). In contrast to Dpl, which accumulates on the apical surface, PrPc is targeted selectively to the basolateral side in Madin-Darby canine kidney cells. An extensive deletion and domain swapping analysis revealed that the internal hydrophobic domain (HD) of PrP (amino acids 113?133) confers basolateral sorting in a dominant manner. PrP mutants lacking the HD are sorted apically, while Dpl chimeras containing the HD of PrP are directed to the basolateral membrane. Furthermore, a pathogenic PrP missense mutation within the HD leads to aberrant apical sorting of PrP as well.
AD Armgard Uelhoff aus dem Adolf-Butenandt-Institut, der Universität München, Lehrstuhl für Stoffwechselbiochemie
SP deutsch
PO Deutschland