NR ATPY
AU Vorberg,I.; Portis,J.; Raines,A.; Meade-White,K.; Gardner,D.; Favara,C.; Priola,S.A.
TI Marginal effect of phagocyte NADPH oxidase and inducible nitric oxide synthase on scrapie incubation times
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-35
PT Konferenz-Poster
AB Oxidative stress has been proposed as one of several pathogenic processes underlying neurodegenerative diseases and has been implicated in the disease development of transmissible spongiform encephalopathies (TSEs). We have studied the role of the reactive oxygen and reactive nitrogen intermediates produced by phagocyte oxidase (Phox) and inducible nitric oxide synthase (iNOS) in TSEs. Here we demonstrate that mice deficient in iNOS are fully susceptible to intracerebral scrapie infections. Moreover, mice with a combined deficiency of iNOS and the gp91 component of phagocyte oxidase also succumbed to scrapie disease. Double deficiency in iNOS/Phox resulted in a slight but statistically significant increase in incubation time with one of the two scrapie strains tested compared to wild-type controls. These results suggest that formation of reactive oxygen species and reactive nitrogen intermediates by Phox and iNOS only marginally influences disease incubation times in TSEs. Differences in the pathology of iNOS and iNOS/Phox deficient mice compared to control mice infected with mouse-adapted scrapie strains will be discussed.
AD I.Vorberg, Institute of Virology; Technical University of Munich; Munich, Germany; J.Portis, A.Raines, K.Meade-White, D.Gardner, C.Favara, S.A.Priola, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Montana, USA
SP englisch
PO Deutschland