NR ATOW
AU Ligios,C.; Cancedda,M.G.; Saba,M.; Santucciu,C.; Maestrale,C.; Agrimi,U.; Ru,G.; Di Guardo,G.
TI PrPsc deposition in nervous tissue with no apparent lymphoid tissue involvement in Sarda breed sheep affected with natural scrapie.
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-07
PT Konferenz-Poster
AB The present study reports the involvement of nervous and lymphoid tissues in Sarda breed sheep naturally affected with preclinical and clinical scrapie. Our investigation was carried out on 1,024 clinically healthy Sarda breed sheep belonging to 8 scrapie-affected flocks. A group of 47 sheep clinically affected with natural scrapie were also included in the study. Nervous and lymphoid tissues of these sheep were submitted to appropriate immunohistochemistry (IHC) and Western-blot (WB) protocols for PrPsc detection. A total of 69 out of the 1,024 clinically healthy sheep were found to be scrapie infected, since PrPsc was detected, by means of both IHC and WB, in the CNS (obex region) and, by means of IHC alone, within enteric nervous system (ENS) plexuses from 60 of the above sheep. Furthermore, IHC and WB yielded evidence of PrPsc deposition only in lymphoid tissues from the remaining 9 out of the 69 clinically healthy sheep. PrPsc was also detected, by IHC and WB, in the CNS as well as, by IHC alone, in ENS plexuses from all the 47 clinically affected sheep. Nevertheless, 18 out of the 69 clinically healthy animals (26%, 17 ARQ/ARQ and 1 ARQ/AHQ sheep), along with 3 ARQ/ARQ sheep (6%) of the clinically affected group, showed no IHC nor WB evidence of PrPsc in a number of lymphoid tissues. The present study demonstrates, in ARQ/ARQ and ARQ/AHQ Sarda breed sheep affected with natural scrapie, dual CNS and ENS PrPsc deposition, opposed in a number of cases by an apparent lack of lymphoid tissue involvement.
IN Untersuchungen an sardischen Schafen zeigen, dass man sich auch nicht darauf verlassen kann, bei Scrapie-infizierten Schafen PrPsc in lymphoiden Geweben nachweisen zu können. Bei 26% der präklinisch diagnostizierten Schafe und bei 6% der bereits an Scrapie erkrankten Schafe konnten die Autoren immunhistochemisch und per Western blot PrPsc nicht in lymphoiden Geweben, sondern nur im Obex nachweisen.
AD C.Ligios, M.Saba, C.Santucciu, C.Maestrale, Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy; M.G.Cancedda, Istituto Zootecnico-Caseario della Sardegna, Olmedo, Italy; U.Agrimi, Istituto Superiore di Sanità, Dipartimento di Sanità Alimentare ed Animale, Rome, Italy; G.Ru, CEA, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy; G.Di Guardo, Università degli Studi di Teramo, Facoltà di Medicina Veterinaria, Dipartimento di Scienze Biomediche Comparate, Teramo, Italy
SP englisch
PO Deutschland