NR ATNA
AU Peletto,S.; Colussi,S.; Riina,M.V.; Corvonato,M.; Corvonato,R.; Gennero,S.; Ferroglio,E.; Orusa,R.; Caramelli,M.; Acutis,P.L.
TI Prion protein gene polymorphisms in Italian wild ruminants
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-26
PT Konferenz-Poster
AB Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) occurring in North American cervids. The possibility of transmission to humans or traditional domestic livestock is of concern. Until now, there is no evidence that TSE (BSE, CWD or scrapie) exists in wild ruminants in Italy or any other European country. However, intracerebral inoculation of elk with sheep scrapie agent have been shown to cause a spongiform encephalopathy. Prion protein (PrP) gene alleles associated with susceptibility to TSEs have been identified in sheep and humans. Sequence analysis of the PrP gene from Rocky Mountain elk showed only one amino acid change (Met to Leu at codon 132) and homozygosis for Met at codon 132 were over-represented in both free-ranging and farm-raised CWD-affected elk when compared to unaffected control groups. In addition, PrPgene polymorphisms encoding amino acid changes were identified in white tailed deer, with substitutions at residues 95 (Q->H), 96 (G->S) or 116 (A->G), each with serine (S) at residue 138 (N->S). Amino acid variation at codon 226 (E->Q) has been described in Chinese domestic sikadeer. In this study we describe nucleotide sequence variation in the PRNP locus of Italian red deer (Cervus elaphus elaphus) (n=100), chamois (Rupicapra rupicapra) (n=100) and roe deer (Capreolus capreolus) (n=100). The PrP gene of the studied wild ungulates encodes for 256 amino acids prion protein with 5 octapeptide repeats in the N-terminal region. The PRNP in roe deer and chamois is highly conserved. New coding polymorphisms have been identified in red deer, two of which are novel (G59S, T98A). Sequence alignment and phylogenetic analysis of the PRNP sequences show a higher similarity between red deer and roe deer and between sheep, goat and chamois. Futhermore these two groups are more similar to each other than to cattle, which has the most divergent protein among the considered species.
AD S.Peletto, S.Colussi, M.V.Riina, M.Corvonato, R.Corvonato, S.Gennero, M.Caramelli, P.L.Acutis, CEA (TSE National Reference Center), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Italy; E.Ferroglio, Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Facoltà di Medicina Veterinaria, Italy; R.Orusa, CERMAS ( National Reference Center for Wildlife Disease), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Sezione di Aosta, Italy
SP englisch
PO Deutschland
EA pdf-Datei, TIFF-Datei sowie Bild 1, Bild 2, Bild 3, Bild 4