NR ATMW
AU Schettler,E.; Steinbach,F.; Blasche,T.; Gerst,K.; Kaps,I.; Risch,K.; Streich,W.J.; Frölich,K.
TI Risk Analysis of Transmissible Spongiform Encephalopathies (TSE) in Cervids from Germany
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-22
PT Konferenz-Poster
AB
Germany has one of the largest deer populations within Europe of which around 19,000 tons of venison are traded and consumed each year. So far, it is not known whether transmissible spongiform encephalopathies (TSE) are present in European deer. Theoretically, a transmission of prion diseases such as chronic wasting disease (CWD), bovine spongiform encephalopathy (BSE) or scrapie to European cervids is possible. To estimate the risk of TSE for cervids in Germany 7,056 free-living cervids were tested between 2002 and 2005 by Bio-Rad ELISA. The samples originated from roe deer (Capreolus capreolus; n=4250), red deer (Cervus elaphus elaphus; n=1416) and fallow deer (Dama dama; n=1390) from throughout Germany. Additionally, various commercial rapid BSE tests were compared regarding their specificity and sensitivity for TSE in cervids. This study represents by far the largest active survey on TSE in European wildlife.
The main objective was to determine the TSE prevalence in cervids from Germany choosing a detection limit of > 0,5% with a 95% probability. To consider several risk factors, such as BSE- and scrapie prevalence in life stock, clinical suspects or captive cervids that fed on concentrates or milk powder, a stratified sampling procedure was set up. No protease resistant prion protein (PrPres) was detected in any of the examined brain and lymph node samples and therefore this study provides no evidence that TSE is present in German cervids. Results regarding the comparison of various rapid tests show that Prionics Western blot, Prionics LIA, and BioRad BSE ELISA all detect cellular prion protein (PrPc) of German deer species as well as PrPres of CWD-positive cervids. However, significant differences were observed regarding sensitivity of the tests. In conclusion, the existence of TSE in cervids from Germany and a risk of TSE transmission to humans resulting from the consumption of their venison are rather unlikely.
Supported by the BMBF grant 0312869
IN Die Autoren untersuchten mit dem Bio-Rad ELISA in Deutschland 4.250 Rehe, 1.416 Rothirsche und 1.390 Stück Damwild. Es gab kein einziges postives Schnelltestergebnis.
AD E.Schettler, F.Steinbach, T.Blasche, W.J.Streich, K.Frölich, Institute for Zoo- and Wildlife Research, Berlin, Germany; K.Gerst, K.Risch, Landesveterinär- und Lebensmitteluntersuchungsamt Mecklenburg Vorpommern, Rostock, Germany; I.Kaps, Cenas AG, Kulmbach, Germany
SP englisch
PO Deutschland