NR ATMO
AU Balbus,N.; Humeny,A.; Kashkevich,K.; Henz,I.; Fischer,C.; Becker,C.M.; Schiebel,K.
TI DNA-polymorphisms of the prion doppel gene region in four different German cattle breeds and cows tested positive for bovine spongiform encephalopathy
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-14
PT Konferenz-Poster
AB Polymorphisms of the prion protein gene, PRNP, have been shown to influence the susceptibility/resistance to prion infections in human and sheep. In addition, the T174M polymorphism within the flanking prion doppel gene (PRND) was discussed to be involved in susceptibility to sporadic Creutzfeldt-Jakob disease. To study a possible influence of DNA polymorphisms of the bovine PRND gene in BSE, previously identified and newly isolated DNA polymorphisms were genotyped in all available German cattle, tested positive for BSE. Genotypes and calculated haplotypes were compared to breeding bulls serving as controls. Analysis of the four major breeds Schwarzbunt (Holstein Friesian), Rotbunt (Holstein Red), Fleckvieh (Simmental) and Braunvieh (Swiss Brown) resulted in the isolation of the previously known polymorphisms R50H and R132Q and two novel synonymous single nucleotide polymorphisms (SNPs) C4820T and A5063T. Comparative genotype and haplotype analysis of BSE and control animals revealed a significantly different distribution of polymorphisms C4815T and R132Q in Fleckvieh animals, but not in the other breeds tested. No association to BSE susceptibility was detectable for polymorphisms R50H and A5063T.
AD N.Balbus, A.Humeny, K.Kashkevich, I.Henz, C.-M.Becker, K.Schiebel, Universität Erlangen-Nürnberg, Institut für Biochemie, Emil-Fischer-Zentrum, Erlangen, Germany; C.Fischer, Universität Heidelberg, Institut für Humangenetik, Heidelberg, Germany
SP englisch
PO Deutschland