NR ATMD
AU Green,R.B.; Sallis,R.E.; Simonini,S.; Horrocks,C.L.; Spiropoulos,J.; Ryder,S.
TI Can BSE be identified in a BSE/scrapie mix, using mouse lesion profiling techniques?
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Genetics, strains and emerging problems GEN-03
PT Konferenz-Poster
AB
Studies are underway in the United Kingdom to try to establish if BSE is present in the national sheep flock. It would be impractical to strain type every individual case of scrapie so if BSE can be isolated when mixed with scrapie, the testing of pools of scrapie positive sheep brains would enable large numbers to be screened for the presence of BSE.
Mouse bioassay techniques are able to identify low levels of BSE on primary isolation, when on a negative background. BSE has a distinctive lesion profile when inoculated into mice, which is distinct from any scrapie lesion profile. This study was undertaken to investigate if BSE could be identified when present in a pool of scrapie brains, consisting of ovine derived BSE at - 100, 50, 10, 2, 1, and 0 %- in scrapie brain.
This poster presents the latest findings based on data from completed mouse bioassays for three mouse strains, including lesion profiles, incubation period, immunohistochemical reaction product distribution, and some cluster analysis.
All the BSE / scrapie mixtures and the pure scrapie brain presented similar profiles to each other, and these differed from the lesion profiles of the 100% BSE transmissions in both height and shape.
A cluster analysis grouped all the mixed and the pure scrapie together, and grouped them with scrapie from our archive. The 100% BSE was grouped tightly with archived BSE derived from ovine, bovine and other sources.
These results suggest that BSE can not be detected on primary isolation in mice, when mixed with an excess of scrapie. It also suggests that the accidental contamination of a scrapie infected brain during removal or subsequent handling is unlikely to compromise the lesion profiling or the diagnosis of scrapie.
[We acknowledge the statistical analysis carried out by Ian Dunbar, Helen Wilkinson and Eleanor Baker, of Risk Solutions]
AD R.B.Green, R.Sallis, S.Simonini, C.L.Horrocks, J.Spiropoulos, S.Ryder, DEFRA, VLA Weybridge, UK
SP englisch
PO Deutschland