NR ATLY
AU Clarke,J.; Cook,J.; Dobson,C.; Stumpf,R.
TI Evaluation of an automated tissue disruption system for use with a rapid TSE assay
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-62
PT Konferenz-Poster
AB
The Enfer TSE Version 2.0 assay is an approved, qualitative immunoassay for the detection of PrPsc in central nervous tissue of cattle and small ruminants. As with most rapid TSE assays, tissue disruption and homogenisation of the samples is a labour intensive and rate limiting step.
Homogenisation by means of a stomacher allows the processing of two samples and takes 2 minutes to perform. Development of a semi automated homogenisation device (Enfer Tissue Disruption System (ETDS)) now allows the simultaneous processing of eight samples and complete homogenisation in 10 seconds and, in contrast to the stomacher bag, facilitates removal of the resulting homogenate by means of an automated pipettor.
This study was undertaken to optimise homogenisation conditions and to verify that performance for the two methods of homogenisation are equivalent.
Data is presented to show that 10 seconds is optimal for homogenisation time and that in terms of sensitivity and specificity the ETDS produces results that are comparable or better than those produced by the stomacher. Relative laboratory throughputs are also compared for the two methods of homogenisation and show an increase in output of up to 15 fold for the ETDS.
AD J.Clarke, Enfer Scientific Limited, Boxer House, Unit 4, Newbridge Industrial Estate, Newbridge, Co. Kildare, Ireland; J.Cook, C.Dobson, Abbott-Murex, Central Road, Temple Hill, Dartford, Kent DA1 5LR, United Kingdom; R.Stumpf, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064-6013
SP englisch
PO Deutschland