NR ATKF
AU Gough,K.C.; Owen,J.P.; Kilpatrick,J.B.; Terry,L.A.; Jackman,R.; Whitelam,G.C.
TI The development of molecular strain typing assays for ovine TSEs
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Diagnosis DIA-17
PT Konferenz-Poster
AB
It is widely assumed that differences in the conformation of the PrPsc prion protein molecule impart the ability of the TSE agent to exist as differing strain types which can be characterised at present only by bioassay. Such protein structural variations will also influence the way that the PrPsc molecule interacts with other proteins, for example enzymes and antibodies and these protein-protein interactions may be exploited in the development of in vitro, biochemical assays that can distinguish among different TSE strains. The major policy issue that would be addressed by such diagnostic assays is whether the BSE agent has infected the sheep population.
Here, we have studied the potential for the combined use of bioactive proteins in recognising different conformers of PrPsc. This has resulted in the development of assays in which TSE strains (experimental BSE and natural scrapie) can be distinguished in ovine samples. The assays include digestion with the protease thermolysin followed by Western blot analysis using N-terminal-specific anti-PrP monoclonal antibodies. Details of such assays are reported and their potential application discussed.
AD K.C.Gough, J.P.Owen, J.B.Kilpatrick, ADAS UK Ltd., UK; L.A.Terry, R.Jackman, Veterinary Laboratory Agency, UK; G.C.Whitelam, University of Leicester, UK
SP englisch
PO Deutschland