NR ATJD
AU Willis,M.P.; Lipscomb,I.P.; Hassall,G.; Perry,V.H.; Keevil,C.W.
TI Cleaning of prion-infected tissue on stainless steel surfaces using Radio Frequency-generated gas plasmas
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-10
PT Konferenz-Poster
AB
Prions, transmissible agents that cause neurodegenerative conditions of the central nervous system, are conformational isomers of the host protein PrPc. This agent is recognised as being particularly resistant to standard methods of inactivation and decontamination such as autoclaving at 121° for 15 minutes, UV irradiation and many gaseous disinfectants. Stainless steel instruments have been shown to retain Transmissible Spongiform Encephalopathy (TSE) infectivity even after formaldehyde treatment. Therefore the risk of transmitting prion disease iatrogenically via surgical instruments is of great current concern.
Here we report on the application of Radio Frequency-generated gas plasma and a novel microscopic technique; Episcopic Differential Interference Contrast-Epifluorescence (EDIC/EF), in conjunction with a sensitive protein stain (SYPRO Ruby) to assess the effect of temperature and gas mixture content on the cleaning efficacy on surgical stainless steel surface bound contamination. Results indicate that the application of plasma cycling in combination with a cooling system enhances the ability of the plasma to remove ME7 Scrapie-infected brain material from the surface of stainless steel, and this is demonstrated preferentially in an oxygen-enriched environment. The gas plasma processing technique is ideally suited for use as part of a hospital sterilisation procedure
IN Mittels episkopischer differenzieller Interferenz-Kontrastmikroskopie und einem Proteinmarkierungsmittel untersuchten die Autoren wie Wirkung von Gasplasmen auf Verunreinigungen von Stahloberflächen mit ME7-Scrapie-Hirn. Sie meinen, dass sich mit Radiowellen erzeugte Gasplasmen in Sauerstoff-angereicherter Umgebung gut für die Krankenhaussterilisation eigne.
AD M.P.Willis, I.P.Lipscomb, C.W.Keevil, Environmental Healthcare Unit, University of Southampton, UK; V.H.Perry, CNS Inflammation Group, University of Southampton, UK; G.Hassall, Oxford Instruments Plasma Technology, UK
SP englisch
PO Deutschland