NR ATIU
AU Baxter,H.C.; Campbell,G.A.; Whittaker,A.G.; Gibbard,L.; Bountiff,L.
TI Radio frequency gas-plasma treatment as a method for decontamination of reprocessed surgical instruments: validation of the removal of scrapie infectivity from stainless steel.
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Decontamination DEC-01
PT Konferenz-Poster
AB
Residual contamination on reprocessed surgical instruments is a significant problem in terms of the transmission of nosocomial infections and of iatrogenic CJD. It is well established that TSE infectivity, which is highly resistant to conventional methods of deactivation, can be transmitted by contaminated stainless steel. With the recent reporting of transmission of variant CJD by blood transfusion ensuring that there is no risk of cross-contamination in surgery from patients in an asymptomatic phase of the disease highlights this problem. It is now increasingly important that new decontamination methods be evaluated for effective removal of protein residues from surgical instruments. In our view, the most effective generic approach would be the routine removal of all traces of organic residues from surgical instruments.
As part of an interdisciplinary group investigating this problem, we are currently examining radiofrequency gas-plasma as a method of removing the residual contaminants from surgical instruments.
We examined stainless steel spheres contaminated with the 263K strain of scrapie, and a variety of reprocessed surgical instruments which had been cleaned by a hospital sterile services department, both before and after treatment by a radio frequency gas-plasma. The transmission of scrapie, from the contaminated spheres, was examined by bioassay using hamsters infected by the peripheral route.
We show that radio frequency gas-plasma treatment effectively removes residual organic residues from reprocessed surgical instruments and from experimentally contaminated spheres. Our in vivo testing shows that gas-plasma treatment of the infected spheres eliminates transmission of TSE infectivity.
IN Nach einer Behandlung mit einem durch Radiowellen erzeugten hochenergetischen Gas-Plasma konnten die Autoren an kontaminierten medizinischen Instrumenten keine Kontamination mit dem 263K-Scrapie-Erreger mehr nachweisen.
AD H.C.Baxter, G.A.Campbell, A.G.Whittaker, University of Edinburgh, UK; L.Gibbard, L.Bountiff, Moredun Research Institute, UK
SP englisch
PO Deutschland