NR ATIS
AU Zawlik,I.; Witusik,M.; Hulas-Bigoszewska,K.; Piaskowski,S.; Szybka,M.; Golanska,E.; Liberski,P.P.; Rieske,P.
TI The Prnp expression in PC-12 cells during differentiation
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-50
PT Konferenz-Poster
AB
The Prnp gene encodes the cellular prion protein (PrPc), which has been implicated in diverse types of transmissible neurodegenerative spongiform encephalopathies. PrPc is considered to be a housekeeping gene, which plays pleiotropic role during embryonal development and mediates neuritogenesis by interaction with laminin. PrPc is strongly expressed in neurons suggesting its important role of neuronal function. However, the physiological function of PrPc and the way in which expression of the Prnp gene is regulated is largely unknown.
The present study was performed on cultures of rat pheochromocytoma PC-12 cells in order to investigate the regulation of the Prnp gene expression in relation to differentiation. The mRNA level of Prnp in NGF-treated PC12 cells was increasing up to forth day of differentiation and slightly increased after treatment with demethylation agent 5-Aza-dCyd. The expression of the PrPc protein in NGF-treated PC12 cells increased in forth day of differentiation and did not change after incubation with 5-Aza-dCyd. Discrepancies in terms of Prnp mRNA and protein expression detected during our experiment prompted us to perform methylation analysis of the Prnp promoter using MS-PCR (Methylation specific PCR). However, no methylation of the promoter has been detected. Slight activation of cloned PRNP promoter has been observed using luciferase reporter system in PC12 cells stimulated with NGF and AKT. These data suggest that PrPc may participate in neuronal differentiation of PC12 cells, but do not indicate an essential role of PrPc in this process.
AD I.Zawlik, M.Witusik, K.Hulas-Bigoszewska, S.Piaskowski, M.Szybka, E.Golanska, P.P.Liberski, P.Rieske, Department of Molecular Pathology and Neuropathology, Chair of Oncology; Medical University of Lodz, Poland
SP englisch
PO Deutschland