NR ATHQ
AU Pastore,M.; Chasseigneaux,S.; Britton-Davidian,J.; Catalan,J.; Steverlynck,C.; Casanova,D.; Laplanche,J.L.; Bürkle,A.; Lehmann,S.L.
TI Investigation on the biological basis of prion susceptibility and resistance in N2A neuroblastoma subclones
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Cell Biology of PrPc and PrPsc CELL-22
PT Konferenz-Poster
AB
Cell cultures represent relevant experimental models of prion diseases but, to date, only few models permissive to prion propagation are available. In this context, the mouse neuroblastoma cell line N2a is one of the most commonly used. However, even this cell line is subject to variation in prion propagation efficiency when cultured over passages. Notably, Bosque and Prusiner (J Virol. 2000 74:4377-86) isolated N2a subclones that featured high susceptibility or resistance to prion infection.
A multidisciplinary program, combining cell biology, proteomic and genomic approaches, was used to investigate the biological basis of prion susceptibility in different susceptible and resistant N2a subclones (Zhang Y et al., 2002. Histochem J. 34:357-63). We looked at the expression, trafficking, processing of PrP, as well as at the biology of other relevant proteins including putative PrP receptors.
Interestingly, karyotype analyses showed that the number of chromosomes varied among different subclones. It remains to be determined if resistance and susceptibility to prion infection are related to this finding.
AD Manuela Pastore, Danielle Casanova, Sylvain Lehmann, Institut de Génétique Humaine UPR 1142, France; Stéphanie Chasseigneaux, Jean-Louis Laplanche, Hôpital Lariboisière, France; Janice Britton-Davidian, Josette Catalan, Université Montpellier II, France; Céline Steverlynck, Faculté de Medicine St Antoine, France; Alexander Burkle, University of Konstanz, Germany
SP englisch
PO Deutschland