NR ASWX
AU Hui,Z.; Minamiguchi,K.; Doi,H.; Kinoshita,N.; Kanouchi,H.; Oka,T.
TI Recombinant alkaline serine protease II degrades scrapie isoform of prion protein
QU In Vitro Cellular and Developmental Biology. Animal 2004 Sep-Oct; 40(8-9): 293-6
PT journal article
AB An efficient Escherichia coli expression system for the production of mature-type alkaline serine protease II (mASP II) has been constructed. Complementary deoxyribonucleic acid-encoding mASP II was inserted into the inducible bacterial expression vector pGE-30. After introduction into E. coli, the plasmid was expressed by isopropyl-1-thio-beta-D-galactopyranoside, and the recombinant product was purified using a Ni-nitrilotriacetic acid column. The purified product had the expected NH2-terminal sequence and showed a scrapie isoform of prion protein-degrading activity using hamster scrapie 263K prions as a substrate.
MH Animals; Bacterial Proteins/genetics/metabolism; Escherichia coli/genetics/metabolism; Hamsters; Molecular Sequence Data; PrPsc Proteins/*metabolism; Protein Isoforms/*metabolism; Recombinant Proteins/genetics/metabolism; Serine Endopeptidases/genetics/*metabolism
AD Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
SP englisch
PO USA