NR ASTL
AU Gellermann,G.P.; Appel,T.R.; Tannert,A.; Radestock,A.; Hortschansky,P.; Schroeckh,V.; Leisner,C.; Lütkepohl,T.; Shtrasburg,S.; Röcken,C.; Pras,M.; Linke,R.P.; Diekmann,S.; Fändrich,M.
TI Raft lipids as common components of human extracellular amyloid fibrils
QU Proceedings of the National Academy of Sciences of the United States of America 2005 May 3; 102(18): 6297-302
IA http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1088351
PT journal article
AB Amyloid fibrils are fibrillar polypeptide aggregates from several degenerative human conditions, including Alzheimer's and Creutzfeldt-Jakob diseases. Analysis of amyloid fibrils derived from various human diseases (AA, ATTR, Abeta2M, ALlambda, and ALkappa amyloidosis) shows that these are associated with a common lipid component that has a conserved chemical composition and that is specifically rich in cholesterol and sphingolipids, the major components of cellular lipid rafts. This pattern is not notably affected by the purification procedure, and no tight lipid interactions can be detected when preformed fibrils are mixed with lipids. By contrast, the early and prefibrillar aggregates formed in an AA amyloid-producing cell system interact with the raft marker ganglioside-1, and amyloid formation is impaired by addition of cholesterol-reducing agents. These data suggest the existence of common cellular mechanisms in the generation of different types of clinical amyloid deposits.
MH Amyloid/*analysis; Amyloidosis/*metabolism; Animals; Chromatography, Thin Layer; Comparative Study; Humans; Immunohistochemistry; Membrane Microdomains/*metabolism; Mice; Monocytes/metabolism; Research Support, Non-U.S. Gov't; Spleen/metabolism
AD Gerald P. Gellermann, Thomas R. Appel, Astrid Tannert, Anja Radestock, Christian Leisner, Tim Lütkepohl, Stephan Diekmann, Marcus Fändrich (fandrich@imb-jena.de), Institut für Molekulare Biotechnologie, Beutenbergstrasse 11, 07745 Jena, Germany; Peter Hortschansky, Volker Schroeckh, Leibniz-Inititut für Naturstoff-Forschung und Infektionsbiologie, Hans-Knöll-Institut, Beutenbergstrasse 11, 07745 Jena, Germany; Shmuel Shtrasburg, Mordechai Pras, Heller Institute of Medical Research, Tel Hashomer 52621, Israel; Christoph Röcken, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, Germany; Reinhold P. Linke, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
SP englisch
PO USA