NR ASNU
AU Schütz,E.; Iakoubov,L.; Wemheuer,W.; Urnovitz,H.B.; Schulz-Schaeffer,W.J.; Brenig,B.
TI Serum nucleic acids directly correlate to BSE risk
QU Presented at the 29th International Conference on Animal Genetics, Tokyo, Japan. 74.4-9902072-1-1
IA http://www.chronixbiomedical.com/dw/isag_posternoshadow.pdf
PT Poster
AB Etiopathologic and immune responses to prions, the agents associated with the transmissible spongiform encephalopathy (TSE) diseases, such as scrapie in sheep and bovine spongiform encephalopathy (BSE) or mad cow disease remain unclear. Germline mutations in the prion coding region have been described in scrapie/sheep but yet to be identified in BSE/cows. Our analysis of microvesicular associated serum circulating nucleic acids (CNA) shows a distinct pattern of repetitive nucleic acids associated with prion-positive cattle and their associated feeding cohorts. Using a differential display PCR test of CNA, a study was conducted on confirmed prion positive cows (N=4), 15 at-risk feeding cohorts of confirmed prion positive cows (N=207) and randomly selected cattle from a local slaughterhouse (N=908). Gene amplification detected unique CNA profiles in all 15 confirmed prion positive associated cohorts with reactive patterns of at least 33% (mean=65%) of the 207 total cohort animal sera tested. In contrast, only 0.55% of controls from normal herds and randomly selected cattle from slaughterhouse were reactive (p<0.001). The unique CNA profiles significantly associated with BSE at-risk cows support the approach of using a CNA PCR ante mortem test for identifying at-risk herds in the assistance of BSE eradication programs.
ZR 4
AD Ekkehard Schütz, Leonid Iakoubov, Howard B. Urnovitz, Chronix Biomedical, Göttingen, Germany; Wilhelm Wemheuer, Bertram Brenig, Institute of Veterinary Medicine, University Göttingen, Germany; Walter Schultz-Schaeffer, Department of Neuropathology, University Clinics, Göttingen, Germany
SP englisch
PO Deutschland