NR ASKP
AU Moya,K.L.; Hässig,R.; Breen,K.C.; Volland,H.; Di Giamberardino,L.
TI Axonal transport of the cellular prion protein is increased during axon regeneration
QU Journal of Neurochemistry 2005 Mar; 92(5): 1044-53
PT journal article
AB The cellular prion protein, PrPc, is a glycosylphosphatidylinositol-anchored cell surface glycoprotein and a protease-resistant conformer of the protein may be the infectious agent in transmissible spongiform encephalopathies. PrPc is localized on growing axons in vitro and along fibre bundles that contain elongating axons in developing and adult brain. To determine whether the growth state of axons influenced the expression and axonal transport of PrPc, we examined changes in the protein following post-traumatic regeneration in the hamster sciatic nerve. Our results show (1) that PrPc in nerve is significantly increased during nerve regeneration; (2) that this increase involves an increase in axonally transported PrPc; and (3) that the PrPc preferentially targeted for the newly formed portions of the regenerating axons consists of higher molecular weight glycoforms. These results raise the possibility that PrPc may play a role in the growth of axons in vivo, perhaps as an adhesion molecule interacting with the extracellular environment through specialized glycosylation.
MH Animals; Axonal Transport/*physiology; Blotting, Western/methods; Cerebral Cortex/metabolism; Comparative Study; Dose-Response Relationship, Drug; Glucuronidase/pharmacology; Glycosylation; Hamsters; Male; Nerve Crush/methods; Nerve Degeneration/metabolism/physiopathology; Nerve Regeneration/*physiology; Neuraminidase/pharmacology; PrPc Proteins/drug effects/*metabolism; Research Support, Non-U.S. Gov't; Sciatic Neuropathy/*metabolism/physiopathology; Time Factors
AD Kenneth L. Moya (moya@shfj.cea.fr), Raymonde Hässig, Kieran C. Breen, Commissariat à l'Energie Atomique - Centre National de Recherche Scientifique Unité de Recherche Associeé URA 2210, Service Hospitalier Frédéric Joliot, DRM/DSV, Orsay, France; Kieran C. Breen, Section of Psychiatry and Behavioural Sciences, Division of Pathology and Neuroscience, University of Dundee Medical School, Ninewells Hospital, Dundee, UK; Hervè Volland, CEA, Service de Pharmacologie et d?Immunologie, CEA/Saclay, 91191 Gif-sur-Yvette, France; Luigi Di Giamberardino, Ecole Normale Supérieure, Paris, France
SP englisch
PO England