NR ASKJ
AU Lobao-Soares,B.; Bianchin,M.M.; Linhares,M.N.; Carqueja,C.L.; Tasca,C.I.; Souza,M.; Marques,W.Jr.; Brentani,R.R.; Martins,V.R.; Sakamoto,A.C.; Carlotti,C.G.Jr.; Walz,R.
TI Normal brain mitochondrial respiration in adult mice lacking cellular prion protein
QU Neuroscience Letters 2005 Mar 3; 375(3): 203-6
PT journal article
AB Cellular prion protein (PrPc) gene (Prnp) null mice (Prnp0/0) show higher sensitivity to seizures, enhanced brain oxidative stress, and their neurons exhibit higher excitability "in vitro". Mitochondrial respiration is a useful parameter for the determination of cellular metabolic rate and it is a major source of reactive oxygen species (ROS). In the present study, we investigated the mitochondrial function of different brain areas of Prnp0/0 adult mice and then compared this to normal control animals. Baseline mitochondrial respiration (stages 3 and 4), respiratory control ratio (RCR) and membrane potential were evaluated in the neocortex, entorhinal cortex, hippocampus, and cerebellum. No differences in these parameters were detected between Prnp0/0 and wild-type mice. Thus, we concluded that baseline mitochondrial respiration might not be directly related with the higher oxidative stress previously observed in brains from Prnp0/0 mice.
MH Animals; Brain/anatomy & histology/*physiology; Cell Respiration/physiology; Comparative Study; Membrane Potentials/physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Mitochondria/*physiology; PrPc Proteins/*deficiency; Research Support, Non-U.S. Gov't; *Respiration; Spectrometry, Fluorescence/methods
AD Departamento de Neurologia, Psiquiatria e Psicologia Medica, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil.
SP englisch
PO Irland