NR ASKB
AU Kim,B.H.; Kim,J.I.; Choi,E.K.; Carp,R.I.; Kim,Y.S.
TI A neuronal cell line that does not express either prion or doppel proteins
QU Neuroreport 2005 Apr 4; 16(5): 425-9
PT journal article
AB Prions have been extensively studied since they represent a new class of infectious agents, the pathogenic prion protein (PrPsc). However, a central question on the physiological function of the normal prion protein (PrPc) remains unresolved. A cell model which was previously established from Rikn mice (PrP-/-) remains problematic because of its ectopic expression of the doppel (Dpl) which may have a neurotoxic effect. Here we established neuronal cell lines from Zürich I (PrP-/-) which do not express Dpl protein and ICR mice (PrP+/+) by transfecting with plasmid encoding for the large T antigen of SV40. The transformed cells have shown neuronal characteristics and, thus, these cell lines may provide a useful model to explore the function of neuronal PrPc.
MH Animals; Blotting, Western/methods; Cell Count/methods; Cell Cycle/physiology; Cell Line, Transformed/metabolism; Cell Size; Cells, Cultured; Comparative Study; Embryo; Fluorescent Antibody Technique/methods; Hippocampus/*cytology; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Mice, Knockout; Microtubule-Associated Proteins/metabolism; Neurons/*metabolism; Phosphopyruvate Hydratase/metabolism; PrPc Proteins/genetics/*metabolism; Prions/*metabolism; RNA, Messenger/biosynthesis; Research Support, Non-U.S. Gov't; Reverse Transcriptase Polymerase Chain Reaction/methods; Time Factors
AD Ilsong Institute of Life Science, College of Medicine, Hallym University, Ilsong Building, Anyang, Kyonggi-do 431-060, South Korea.
SP englisch
PO England