NR ASEO
AU Soler-Federsppiel,B.S.; Cras,P.; Gheuens,J.; Andries,D.; Lowenthal,A.
TI Human gamma gamma-enolase: two-site immunoradiometric assay with a single monoclonal antibody.
QU Journal of Neurochemistry 1987 Jan; 48(1): 22-8
PT journal article
AB A monoclonal antibody (mAb), termed BBS/NC/VI-H14 (H14), that reacts with the human enzyme gamma gamma-enolase was prepared. It was directed against the gamma-subunit and did not cross-react with the alpha- or beta-subunit. The mAb H14 can be used for quantitative determination of gamma gamma-enolase in a two-site immunoradiometric assay (two-site IRMA). It is also suitable for immunostaining formalin-fixed tissues. The specific identification of gamma gamma-enolase provided by the two-site IRMA with H14 is discussed in relation to the cellular distribution of this protein.
IN Der monoklonale Antikörper BBS/NC/VI-H14 erkennt spezifisch das menschliche Enzym gamma-gamma-Enolase und nicht die Untereinheiten alpha oder beta.
MH Animal; Antibodies, Monoclonal/immunology; Antibody Specificity; Brain/*enzymology; Guinea Pigs; Histocytochemistry; Human; Immunoassay; Iodine Radioisotopes; Isoenzymes/*analysis/cerebrospinal fluid/immunology; Phosphopyruvate Hydratase/*analysis/cerebrospinal fluid/immunology; Support, Non-U.S. Gov't
SP englisch
PO USA