NR ARUT
AU Kataoka,N.; Nishimura,M.; Horiuchi,M.; Ishiguro,N.
TI Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido
QU Journal of Veterinary Medical Science 2005 Mar; 67(3): 349-51
PT journal article
AB Surveillance of chronic wasting disease (CWD) was conducted by performing Western blot analysis of tissue samples from 136 sika deer (Cervus nippon) killed by hunters in the Tokachi district of Hokkaido Island. No prion protein (PrPsc) associated with CWD was detected in any of the samples. To assess amino acid polymorphisms of the sika deer PrP gene, nucleotide sequencing of the PrP gene was performed. The only amino acid polymorphisms detected were 3 silent mutations at nucleotide positions 63, 225 and 408. These results suggest that sika deer in the Tokachi district are genetically homogeneous, and are not infected with CWD.
MH Amino Acid Sequence; Animals; Blotting, Western/veterinary; Comparative Study; *Deer; Japan/epidemiology; Point Mutation/genetics; *Polymorphism, Genetic; Prions/*genetics; Research Support, Non-U.S. Gov't; Sequence Analysis, DNA/veterinary; Wasting Disease, Chronic/*epidemiology/*genetics
AD Laboratory of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
SP englisch
PO Japan