NR ARQP
AU Kaneider,N.C.; Kaser,A.; Dunzendorfer,S.; Tilg,H.; Patsch,J.R.; Wiedermann,C.J.
TI Neurokinin-1 receptor interacts with PrP(106-126)-induced dendritic cell migration and maturation
QU Journal of Neuroimmunology 2005 Jan; 158(1-2): 153-8
PT journal article
AB Circulating monocytic cells may mediate neuroinvasion in transmissible spongiform encephalopathies by transporting prion protein (PrP) from sites of entry to the nervous system. Factors regulating monocyte-derived dendritic cell (DC) functions in neuronal tissue include neurogenic mediators, but their interactions with prion-infected DCs are unknown. Here, we report that neuropeptides regulate the interaction of DCs exposed to PrP(106-126). Inhibition of neurokinin-1-receptors activation with neurokinin-1-receptor antagonist or antibody attenuates substance P-induced enhancement of DC migration induced by PrP(106-126) and prion-protein-induced DC maturation. Other neuropeptides arrest chemotaxis. Data support a priming role of the neuropeptide substance P in PrP(106-126)-induced migration of DCs involving neurokinin-1-receptors.
MH Antibodies/pharmacology; Antigens, CD80/metabolism; Cell Aging/*drug effects/physiology; Cell Movement/*drug effects; Cells, Cultured; Chemotaxis/*drug effects/physiology; Comparative Study; Dendritic Cells/*drug effects; Dose-Response Relationship, Drug; Drug Interactions; Flow Cytometry/methods; HLA-DR Antigens/metabolism; Humans; Neuropeptides/pharmacology; Peptide Fragments/*pharmacology; Prions/*pharmacology; Receptors, Neurokinin-1/antagonists & inhibitors/immunology/*metabolism; Statistics, Nonparametric
AD Division of General Internal Medicine, Department of Internal Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
SP englisch
PO Niederlande