NR ARFY
AU Browning,S.R.; Mason,G.L.; Seward,T.; Green,M.; Eliason,G.A.J.; Mathiason,C.K.; Miller,M.W.; Williams,E.S.; Hoover,E.; Telling,G.C.
TI Transmission of prions from mule deer and elk with chronic wasting disease to transgenic mice expressing cervid PrP
QU Journal of Virology 2004 Dec; 78(23): 13345-50
PT journal article
AB We generated mice expressing cervid prion protein to produce a transgenic system simulating chronic wasting disease (CWD) in deer and elk. While normal mice were resistant to CWD, these transgenic mice uniformly developed signs of neurological dysfunction approximately 230 days following intracerebral inoculation with four CWD isolates. Inoculated transgenic mice homozygous for the transgene array developed disease after approximately 160 days. The brains of sick transgenic mice exhibited widespread spongiform degeneration and contained abnormal prion protein and abundant amyloid plaques, many of which were florid plaques. Transmission studies indicated that the same prion strain caused CWD in the analyzed mule deer and elk. These mice provide a new and reliable tool for detecting CWD prions.
IN Die Autoren inokulierten intrazerebral unter anderem 7 Wildtyp-Mäuse mit 1%igem Hirnhomogenat CWD-kranker Maultierhirsche, und nach 596 Tagen war noch keine einzige der Empfängermäuse erkrankt. Damit bestätigten die Autoren entsprechende Beobachtungen von Moira Bruce, über die sie die Autoren mündlich in Kenntnis setzte.
MH Animals; Deer; Mice; Mice, Transgenic; PrPsc Proteins/metabolism; Prions/*physiology; Research Support, U.S. Gov't, P.H.S.; Wasting Disease, Chronic/etiology/pathology/*transmission
AD Department of Microbiology, University of Kentucky, 800 Rose St., Lexington, KY 40536, USA
SP englisch
PO USA