NR ARFW
AU Booth,S.A.; Bowman,C.; Baumgartner,R.; Dolenko,B.; Sorensen,G.; Robertson,C.; Coulthart,M.B.; Phillipson,C.; Somorjai,R.
TI Molecular classification of scrapie strains in mice using gene expression profiling
QU Biochemical and Biophysical Research Communications 2004 Dec 24; 325(4): 1339-45
PT evaluation studies; journal article; validation studies
AB Transmissible spongiform encephalopathy strains demonstrate specific prion characteristics, each with specific incubation times, and strain-specific patterns of deposition of the misfolded isoform of prion, PrPsc, in the brains of infected individuals. Different biochemical properties, including glycosylation profiles and the degree of proteinase resistance, have been shown to be strain-specific. However, no relationship between these properties and the phenotypic differences in the subsequent diseases has as yet been determined. Here we explore the utility of gene expression profiles to identify differences in the host response to different strains of prion agent. We identify 114 genes that exhibit significantly different levels of expression in mice infected with three strains of scrapie. These genes represent a pool of genes involved in a strain-specific response to prion disease. We have identified the most discriminatory genes from this list utilizing a wrapper-based feature selection algorithm with external cross-validation.
MH *Algorithms; Animals; Biological Markers/*metabolism; Brain/*metabolism; Comparative Study; Gene Expression Profiling/*methods; Genetic Screening/methods; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins/*metabolism; Oligonucleotide Array Sequence Analysis/methods; Reproducibility of Results; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Scrapie/*classification/genetics/*metabolism; Sensitivity and Specificity
AD Stephanie Booth (Stephanie_Booth@hc-sc.gc.ca), Garrett Sorensen, Catherine Robertson, Michael Coulthart, Clark Phillipsona, Division of Host Genetics and Prion Diseases, National Microbiology Laboratory, 1015 Arlington Street, Health Canada, Winnipeg, Man., Canada R3E 3R2; Stephanie Booth, Michael Coulthart, Department of Medical Microbiology, University of Manitoba, Winnipeg, Man., Canada R3E 0W3; Christopher Bowman, Richard Baumgartner, Brion Dolenko, Rajmund Somorjai, Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Man., Canada R3B 1Y6
SP englisch
PO USA