NR ARCN
AU Steinbach,F.; Schettler,E.; Streich,W.J.; Blasche,T.; Kaps,I.; Meussdoerffer,F.; Gest,K.; Risch,K.; Frölich,K.
TI TSE in German cervids - results of the years 2002-2004
QU TSE-Forum, 4. Kongress - Nationale TSE-Forschungsplattform, Düsseldorf 28.10.-29.10.2004, Vortrag V-04
PT Konferenz-Vortrag
AB
TSE occur as chronic wasting disease (CWD) in north American cervids since more than 30 years. Additionally, scrapie is present in Germany for more than 200 years and since the year 2000, BSE is detected in German cattle. Until now, CWD has been observed in mule deer (Odocoileus hemionus), white tailed deer (Odocoileus virginianus) and Rocky Mountain wapiti (Cervus elaphus nelsoni) only. The incubation period ranges from several months to years and death is inevitable. Until now, there is no information that any TSE (BSE, CWD or scrapie) occurs in cervids from Germany or another European country. Accordingly, the objectives of our study are to (1) determine the prevalence of TSE in deer in Germany and (2) to compare various commercial rapid BSE tests regarding their specificity and sensitivity especially for TSE in cervids.
Within a period of three years approx. 9,000 brain (obex region) and lymph node samples will be examined for TSE by rapid BSE post mortem tests (Bio-Rad Platelia ELISA). The samples originate from roe deer (Capreolus capreolus), red deer (Cervus elaphus elaphus), and fallow deer (Dama dama) aged >= 18 months. Special emphasis will be provided on collecting samples from cervids living in regions where BSE or scrapie has been diagnosed and on individuals that show clinical signs like cachexia or CNS disorders. The specificity and sensitivity of various BSE tests will be analysed using CWD positive tissue from North America and brain samples (without proteinase digestion) of German cervid species.
Prior to the final huting season of 2004-2005, more than 4000 samples of cervids from Germany were tested negative by Bio-Rad ELISA. The samples originated from all over Germany. First results on rapid tests show that the Prionics Western blot, Prionics LIA, and BioRad BSE ELISA detect both proteinase K resistant prion protein (PrPres) of CWD-positive animals and cellular prion protein (PrPc) of German cervid species. There are differences regarding handling and sensitivity of the tests that will be discussed briefly.
AD Steinbach,F.; Schettler,E.; Streich,W.J.; Blasche,T.; Frölich,K., Institute for Zoo and Wildlife Research, Alfred-Kowalke Straße 17, 10315 Berlin, Deutschland; Kaps,I.; Meussdoerffer,F., Cenas AG, E.-C. Baumann Straße 20, 95326 Kulmbach, Deutschland; Gest,K.; Risch,K., Landesveterinär-und Lebensmitteluntersuchungsamt Thierfelder Straße 18, 18059 Rostock, Deutschland
SP englisch
PO Deutschland
OR Tagungsband