NR ARAX
AU Nagyova,J.; Pastorek,J.; Kopacek,J.
TI Identification of the critical cis-acting elements in the promoter of the mouse Prnd gene coding for Doppel protein
QU Biochimica et Biophysica Acta 2004 Sep 17; 1679(3): 288-93
PT journal article
AB Prnd gene encodes a prion-like protein Doppel. To elucidate the transcriptional regulation of the mouse Prnd gene, we performed a study of its 5' flanking region. Serial deletion analysis of the -1863/+27 genomic fragment revealed the region -185/+27 as a core promoter. Cis-acting elements at the -182/-177 (E box) and -108/-104 (CCAAT box) positions were identified as the main activators of the Prnd expression in GC1-spg cells, with NF-Y being the important contributor. The gel-shift experiments confirmed the binding of the transcription factors to -108/-104 NF-Y consensus motif. An intron1 was found to enhance the Prnd expression in bEND.3 endothelial cells, suggesting an involvement of the endothelial-specific enhancer.
MH 5' Flanking Region; Animals; Base Sequence; Binding Sites; Brain/cytology; CCAAT-Binding Factor/metabolism; Cells, Cultured; Endothelial Cells/physiology; Gene Expression Regulation; Male; Mice; Molecular Sequence Data; Organ Specificity; Prions/*genetics/metabolism; *Promoter Regions (Genetics); *Regulatory Sequences, Nucleic Acid; Support, Non-U.S. Gov't; Testis/cytology/physiology
AD Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 845 05, Slovakia.
SP englisch
PO Niederlande