NR AQOT
AU Seabury,C.M.; Derr,J.N.
TI Identification of a novel ovine PrP polymorphism and scrapie-resistant genotypes for St. Croix White and a related composite breed.
QU Cytogenetic and Genome Research 2003; 102(1-4): 85-8
PT journal article
AB Susceptibility to scrapie is primarily controlled by polymorphisms in the ovine prion protein gene (PRNP). Here, we report a novel ovine exon three PRNP polymorphism (SNP G346C; P116), its association with the ovine ARQ allele (P116A136R154Q171), and two new genotypes (PARQ/ARR; PARQ/ARQ) for the St. Croix White (SCW) breed and a related composite (CMP) breed developed for meat production. The (P116) polymorphism occurs between the N-terminal cleavage site and the hydrophobic region of the ovine prion protein, a region which exhibits extreme conservation across mammalian taxa. The relatively high frequency (0.75) of resistant ARR alleles and the absence of ARQ alleles for the SCW ewes used as breeding stock for CMP resulted in significant genic differentiation (P = 0.0123; S.E. = 0.00113). Additionally, the majority of the SCW (66.7%) and CMP (65.4%) sampled possessed genotypes considered resistant or nearly resistant to scrapie and experimental BSE (bovine spongiform encephalopathy.
MH Alleles; Amino Acid Sequence/genetics; Animals; *Breeding; Cattle; Comparative Study; Conserved Sequence/genetics; Exons/genetics; Genotype; Guinea Pigs; Human; Hybridization, Genetic/*genetics; Mice; Molecular Sequence Data; Polymorphism (Genetics)/*genetics; PrPc Proteins/*genetics; Rabbits; Scrapie/*genetics; Sequence Alignment/methods/veterinary; Sheep, Domestic/*genetics; Support, Non-U.S. Gov't
AD Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4467, USA
SP englisch
PO Schweiz