NR AQLY
AU Leucht,C.; Vana,K.; Renner-Muller,I.; Dormont,D.; Lasmezas,C.I.; Wolf,E.; Weiss,S.
TI Knock-down of the 37-kDa/67-kDa laminin receptor in mouse brain by transgenic expression of specific antisense LRP RNA
QU Transgenic Research 2004 Feb; 13(1): 81-5
PT journal article
AB The 37-kDa/67-kDa laminin receptor (LRP/LR) plays a major role in the propagation of PrPsc, the abnormal form of the prion protein. In order to ablate the expression of LRP/LR in mouse brain we generated transgenic mice ectopically expressing antisense LRP RNA in the brain under control of the neuron-specific enolase (NSE) promoter. Hemizygous transgenic mice TgN(NSEasLRP)2 showed a significant reduction of LRP/LR protein levels in hippocampal and cerebellar brain regions. These mice might act as powerful tools to investigate the role of the laminin receptor in scrapie pathogenesis.
MH Animals; Brain/*metabolism; Cell Line; *Gene Expression; Mice/*genetics; Mice, Transgenic; Molecular Weight; Phosphopyruvate Hydratase/genetics; Promoter Regions (Genetics); RNA, Antisense/*metabolism; Rats; Receptors, Laminin/genetics/*metabolism; Research Support, Non-U.S. Gov't
AD Laboratorium für Molekulare Biologie Genzentrum Institut für Biochemie der LMU München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
SP englisch
PO Niederlande