NR AQJK
AU Fernandez Ocana,M.; Neubert,H.; Przyborowska,A.; Parker,R.; Bramley,P.; Halket,J.; Patel,R.
TI BSE control: detection of gelatine-derived peptides in animal feed by mass spectrometry.
QU Analyst 2004 Feb; 129(2): 111-5
PT evaluation studies; journal article
AB The epidemic of bovine spongiform encephalopathy (BSE) is thought to have resulted from feeding scrapie-infected sheep to cattle. This has led to a ban of feeding animals with "processed animal protein"(PAP). We report a novel approach for the mass spectrometric detection of PAP contamination in animal feedstuffs by detecting gelatine, a derivative of the major animal protein collagen. A method was developed to hydrolyse gelatine standards with hydrochloric acid, followed by detection of the derived hydrolytic peptides at m/z 828, 915, 957 and 1044 by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography electrospray ionisation mass spectrometry (LC-ESI-MS-MS). The marker peptides can be detected at concentrations of 100 ng ml(-1) gelatine in water with MALDI. The procedure was adapted to obtain a suitable peptide map of gelatine extracted from spiked feed. The ratio of signal area of the gelatine-derived peptide at m/z 1044 to the internal standard at m/z 556 is shown to relate to the total amount of gelatine present in the sample.
MH Animal Feed/*analysis; Animals; Cattle; Encephalopathy, Bovine Spongiform/*prevention & control; Food Contamination/*analysis; Gelatin/*analysis; Hydrolysis; Spectrum Analysis, Mass/methods; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.
AD Centre for Chemical and Bioanalytical Sciences, Royal Holloway University of London, Egham, UK W20 0EX. raj.patel@rhul.ac.uk
SP englisch
PO England