NR AQHB
AU Calissano,M.; Ensor,E.; Brown,D.R.; Latchman,D.S.
TI Doppel expression is regulated by the Brn-3a and Brn-3b transcription factors
QU Neuroreport 2004 Mar 1; 15(3): 483-6
PT journal article
AB Doppel and the prion protein (PrP) are two related proteins involved in different aspects of neuronal degeneration. While a structural modification of PrP is necessary and sufficient for its toxic effect, the neurotoxicity of Doppel in the Purkinje cells of the cerebellum relies solely on its overexpression. Understanding the Doppel-related neurotoxicity thus involves the analysis of its developmental and transcriptional regulation. Here we report for the first time that Doppel is expressed in the embryonic neurons of mice dorsal root ganglia and spinal cord and that the closely related Brn-3a and Brn-3b transcription factors are involved in its transcriptional regulation.
MH Animals; Blotting, Western; Brain Neoplasms/metabolism; Cell Line, Tumor; Cells, Cultured; DNA-Binding Proteins/*genetics; Ganglia, Spinal/embryology/metabolism; Gene Expression Regulation/physiology; Human; In Situ Hybridization; Mice; Mice, Knockout; Neuroblastoma/metabolism; Prions/*biosynthesis/genetics; RNA, Messenger/biosynthesis/genetics; Reverse Transcriptase Polymerase Chain Reaction; Spinal Cord/embryology/metabolism; Support, Non-U.S. Gov't; Transcription Factors/*genetics; Transfection
AD MMBU, Institute of Child Health, University College London 30 Guilford Street, London WC1N 1EH, UK. m.calissano@ich.ucl.ac.uk
SP englisch
PO England