NR AQEP
AU Grobet,L.; Vandevenne,S.; Charlier,C.; Pastoret,P.P.; Hanset,R.
TI Polymorphism of the prion protein gene in belgian cattle
QU Annales de Médecine Vétérinaire 1994; 138(N8): 581-6
PT Article
AB The prion protein gene seems to play a key role in the pathogenesis of spongiform encephalopathies. Indeed, polymorphisms of the prion protein gene were associated to the susceptibility to natural infection in human and sheep or to different survival times after experimental inoculation with infectious material in sheep, mouse and hamster. Polymorphisms of the bovine prion protein gene were investigated in the present study. No variation was found using hybridization of bovine genomic PrP DNA nor murine PrP cDNA on bovine BamH I, Bgl II, EcoR I, Hae III, Hind III, Hinf I, Msp I, Psf I, Pvu II and Tag I Southern blots. After PCR amplification of a variable region of the bovine PrP gene, two alleles of different molecular weights were detected in different breeds of the belgian cattle population, including Belgian White Blue. The frequency of the A allele (of highest M.W.) was greater than or equal to 0.95 in all groups, whereas the B allele frequency was so low that only one BB homozygote was found among the 135 tested animals. Direct sequencing of the PCR product showed the same insertion/deletion of a 24 bp GC-rich sequence within a stretch of 5 to 6 repeated elements in the PrP coding exon as previously described (Goldmann et al., 1991b). Our data provide the first records of the bovine PrP variability in belgian cattle.
ZR 38
SP französisch